I used Daytrip for a more customized transfer from Vienna to Prague - we visited Mikulov and Kutna Hora on the way, at at a local non-touristy restaurant and learn a lot from our very knowledgeable driver!
Everything went very well! I absolutely recommend Daytrip for a more private, customized and convenient way to travel from one city to another visiting chosen places on the way. It's way better than taking the bus and you decide your itinerary according to your interests and available time. Great people and service!