At peak season, I'd recommend researching what you want to do in Florence, and booking tickets and timeslots in advance whenever possible - it will save you a LOT of queuing time, and potentially, disappointment.
The Duomo climb is no exception as you'll need to purchase a ticket and at the same time, validate the ticket to make a reservation for a time slot to climb. Entirely possible the first available slot will be the NEXT day so don't leave this one until the last minute.
It's well worth the effort though, to see the inner workings of the cathedral's structure, to see the views both inside the dome and across Florence from the top, and probably to lose a few pounds via the climb itself which is a decent workout by anyone's standards! On that note, caution anyone with serious health issues, or who is really afraid of heights or spiral staircases - it's a long way up and really quite hard work. There are hand rails most of the way but they're not everywhere. Equally, the route up and down is separate where possible, but there are sections where you will meet people coming the other way. 'Passing Places' are usually available.
Great experience highly recommended
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