Urbino is not a destination which can be reached from Rome, either quickly or easily.
The "classic" approach from Rome is by Trenitalia train to Pesaro (usually requiring at least one connection, at Falconara Marittima), then the local bus from the Pesaro train station to Urbino: a trip which takes at least FOUR-FIVE HOURS by train just to Pesaro, then another 45 minutes (and 3.40 Euros) by express bus to Urbino!
The fastest train is the late afternoon Trenitalia Frecciabianca from Roma Termini to Pesaro (3 hours, 15 minutes), which nevertheless arrives in Pesaro too late to connect to the bus to Urbino.
So, the train is a poor solution even under the best of circumstances.
THE DIRECT BUS:
Fortunately, ADRIABUS operates a DIRECT bus from Rome's Tiburtina bus depot (300 meters from the Tiburtina bus/metro station) to Urbino, taking four hours, 15 minutes and costing 38 Euros. There are no connections and therefore no missed connections!
Children under the age of 6 ride FREE on the Adriabus to Urbino, as long as not occupying a seat (or whenever there are unoccupied seats).
Luggage is placed in the underfloor compartment of the bus.
ADRIABUS departs from the Tiburtina bus depot from Monday through Saturday at 7:30 and again at 16:00. On Sundays, there is only one bus, at 18:00.
adriabus.eu/media/…20V.pdf (Pesaro/Urbino bus schedules)
CONNECTIONS FROM/TO Rome FCO Airport:
Take the Trenitalia unreserved airport train between Rome FCO Airport and Roma Tiburtina Station for 8 Euros. Travel time is 48 minutes, and trains run every 15 minutes from Monday through Saturday, and every 30 minutes on Sunday.
The distance between the Tiburtina train/metro station and the Tiburtina bus depot is 300 meters.
If you plan to take the train out of Termini Station, there is a different Trenitalia unreserved airport train (the nonstop Leonardo da Vinci train) which takes 32 minutes and runs every 30 minutes. Service is guaranteed even during strikes.
Airport train tickets MUST be validated prior to boarding, in the stamping machines at the head of the departure platform.
Children up to the age of 12 years, 0 days travel free on both airport trains with a paying adult. Extra children pay 50% of the adult fare.
The ADRIABUS departure to Urbino at 16:00 (Monday through Saturday) is workable for many passengers arriving at FCO Airport on flights before 13:30.
If you arrive very early by plane at FCO, you could take the airport train to Termini Station, place your bags in the luggage hold, then visit the stupendous MUSEO NAZIONALE ROMANO across the square (both the Palazzo Massimo alle Terme and the Terme di Diocleziano, using the same ticket). After retrieving your bags, go to Tiburtina metro station by underground metro (1.50 Euros), then walk 300 meters to the Tiburtina bus depot for the bus to Urbino.
There are also inexpensive airport BUSES to Termini for as little as 4 Euros if you don't want to pay 14 Euros for the Trenitalia airport train (but the buses take 60 minutes, instead of just 32 minutes for the train).Edited: 4:33 pm, October 07, 2013