In a town that dates back 500 years, accessibility can be an issue.  Parking is very limited and the best way to get around is walking or biking.  For those with limited mobility, it is better to limit your day's activities or take breaks throughout. Taxis are just a phone call away as well.

The Lightner Museum  - has elevators but no seating throughout to enable soemone to take a rest while walking through the galleries.

St. George Street - many shops, best broken up over several visits.  Many of the stores are not wheelchair (or stroller) accessible due to narrow doorways and cramped displays.   

Trolley Tours (both companies, Old Town Trolley and Ripleys Red Train) - must be able to step up into the trolley.  No wheelchair lift.  Does not offer a kneeling bus so you will need help up into the trolley. 

Castilo de San Marcos - Accesibility on the first floor.  Gun deck is only accesible by stairs.  Steps are wide but do not have safety rail on the right side. 

San Sebastian Winery - Has an elevator.  Tour is wheelchair accesible.  However, tasting room is set up for people to stand at the bar.  Could position yourself at the end of the bar to be able to view the tour guide. 

Some galleries and shops have a wheelchair accessible back door, so if you have your heart set on visiting, perhaps call ahead and ask.