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Tuscany is a pretty safe place for travelers - violent crime is not exactly running rampant through the streets. Like in most tourist areas, the biggest problem you're likely to have is with pickpockets. When on public transportation (i.e., crowded buses), make sure to keep your bags in front of you, securely zipped and just keep an eye about you. In some cities around Italy, gypsies are well known for their pickpocketing skills - they often work in teams.
In cities like Florence, it's probably best to avoid the train station area at night - you'll know it when you see it because it seems a bit more rundown and filled with people just sort of hanging around. Use caution around Santa Maria Novella and Santa Croce. Around the main tourist attractions, however, you should have little trouble because there are always people around day and night.
In other cities and towns, take care when walking alone at night, especially in unfamiliar areas when you are by yourself. Although it can be great fun to go out to a bar and meet the locals, be careful if you decide to party on your own - especially women. You can't always tell if someone's intentions are noble - most locals are friendly and sincere, but there can always be that random one who is interested particularly in meeting travelers who are a bit sauced for reasons that are less than genuine.