They had a couple routes that you could take, Tuckasegee River route from Dillsboro, NC and the Nantahala Gorge route from Bryson City, NC. (Go to their website, gsmr.com, to see current routes, schedules and prices). We went on the Tuckasegee River route since we were near Dillsboro. It was a nice, scenic ride that followed the river and back. We rode in the open car to try and get some good pictures and video. Nothing that spectacular on this route. Many good photo ops were completely obscured by vegetation between the train and the river. Another note about this route, as you near Bryson City, there is a dirt road between the train and the river. This can make it a little dusty depending on traffic.
Overall, it was nice but overpriced as we can get a day pass for Disney for less per person. Others have written that the Nantahala Gorge route from Bryson City is spectacular. We are likely to give this route a try on our next trip in the area.
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